Journal: iScience

Article Title: PGC-1α in the myofibers regulates the balance between myogenic and adipogenic progenitors affecting muscle regeneration

doi: 10.1016/j.isci.2022.105480

Figure Lengend Snippet:

Article Snippet: Mouse monoclonal anti-Integrin-α7 (clone 3C12), PE-Vio770-conjugated , Miltenyi Biotec , Cat#130-102-718; RRID: AB_2652468.

Techniques: Recombinant, Cell Isolation, cDNA Synthesis, SYBR Green Assay, Isolation, Software

Journal: Pharmacological Research

Article Title: A dual acting compound releasing nitric oxide (NO) and ibuprofen, NCX 320, shows significant therapeutic effects in a mouse model of muscular dystrophy

doi: 10.1016/j.phrs.2011.05.003

Figure Lengend Snippet: NCX 320 increases muscle regeneration, myogenic precursor cells and regenerative potential. (A) Number of centronucleated-regenerating fibres quantified in sections of diaphragm and tibialis anterior muscles; (B) number of CD34/α7 integrin-positive cells isolated from tibialis anterior muscles was measured by flow cytometry; (C) expression of the differentiation markers myogenin (Myog) and myosin heavy chain (MHC) in isolated myogenic precursor cells. All parameters were evaluated in mice treated for 8 months with ibuprofen or NCX 320 incorporated into the diet. Animals receiving the same diet without any drug were used as control. Data are expressed as mean ± SEM ( n = 10). * p < 0.05 vs control animals and # p < 0.05 vs ibuprofen-treated animals. (D) Representative images of the immunoblotting experiments.

Article Snippet: Fluorescein isothiocyanate-conjugated CD34 antibody was purchased from AbD Serotec (Oxford, UK) and phycoerythrin-conjugated α7-integrin antibody from MBL (Woburn, MA, USA).

Techniques: Isolation, Flow Cytometry, Expressing, Western Blot

Journal: Development (Cambridge, England)

Article Title: Laminin α2 controls mouse and human stem cell behaviour during midbrain dopaminergic neuron development

doi: 10.1242/dev.172668

Figure Lengend Snippet: Concentration-dependent effects of lm211 are mediated by distinct receptor engagement. (A) Immunostaining of day 28 cultures treated with lm211 (4 μg ml−1) in combination with integrin blocking antibodies and a control condition of lm211 (1 μg ml−1). (B,C) Quantification of proliferation (B) and differentiation (C) following blocking of integrin α-subunit demonstrates that blocking both integrins α6 and α7 reverses the effect of the lm211-rich environment. (D) Images of day 28 cultures treated with laminin isoforms (1 μg ml−1) and dystroglycan blocking antibody or isotype control. (E,F) Quantification of proliferation (E) and differentiation (F) demonstrates that blockade of dystroglycan reverses the gain in mDA neurons seen in response to treatment with lm211 compared with lm111 without effecting proliferation. Unpaired two-tailed t-test; *P<0.0001, n=3. Scale bars: 50 μm in all images.

Article Snippet: Antibodies used were integrin α3 IA3 (R&D MAB1345-SP), integrin α6 GoH3 (R&D MAB13501), integrin α7 6A11 (LifeSpan Biosciences, LS-C179572), dystroglycan IIH6C4 (Millipore, 05-593), mouse-IgM (ThermoFisher, 02-6800), rat-IgG2a (ThermoFisher, 02-9688) and mouse-IgG1 (ThermoFisher, 02-6100).

Techniques: Concentration Assay, Immunostaining, Blocking Assay, Two Tailed Test